环孢菌素A人工抗原的制备

PREPARATION OF CYCLOSPORINE A IMMUNOGEN

在紫外光激发下使环孢菌素A(CsA)与 4 苯甲酰苯甲酸 (BBa)反应 ,生成含活泼羧基的CsA BBa轭合物 ,再在 1 乙基 3 (3 二甲氨基丙基 )碳二亚胺 (EDCI)作用下 ,使之与聚L 赖氨酸 (PL)偶联 ,从而制得了一种新颖的CsA人工抗原 .用UV ,IR和1HNMR谱证实了CsA BBa轭合物的结构 ,偶联效率达平均分子量为 40 0 0 0的PL分子上 13 1～ 17 4个CsA分子 .

Cyclosproine A reacted with 4 benzoylbenzoic acid under ultraviolet radiation to yield the conjugate of CsA BBa, the conjugate was coupled to polylysine (PL) and then the immunogen of CsA was obtained. The constitution of CsA BBa was confirmed by UV, IR and HNMR, the coupling efficiency was determined by UV spectrophotometer. The coupling product had 13 1～17 4 molecules of CsA per molecule of PL whose average molecular weight is 40000