山羊和水牛感染肝片吸虫后的细胞免疫研究

Cellular immune of goats and buffaloes experimentally infected with F.hepatica

本研究采用间接法检测了山羊和水牛人工感染肝片吸虫后外周血液中淋巴细胞增殖能力、自然杀伤 (NK)活性和分泌白细胞介素 2 (IL 2 )的水平。结果表明两组山羊 (分别一次感染 2 0 0或 5 0 0个囊蚴 )感染后的淋巴细胞转化率呈显著下降趋势 (P <0 .0 5 ;或P <0 .0 1) ;水牛 (每头慢性感染 12 0 0个囊蚴 )的淋巴细胞转化率呈显著升高趋势 (P <0 .0 5 )。未感染水牛的NK活性比山羊的高。感染水牛的NK活性无显著的变化 ;感染山羊的NK活性有两次高峰。未感染水牛的IL 2水平比山羊的高。感染水牛和山羊的淋巴细胞分泌IL 2的水平都有所升高 (P <0 .0 5 ;或P <0 .0 1)。而感染水牛和山羊的淋巴细胞在体外对重组牛IL 2 (r IL 2 )的增殖反应降低 ,这表明还存在某些因素影响着IL 2参与到TH1细胞群保护性免疫中来。总之 ,水牛比山羊对肝片吸虫的天然免疫力强。肝片吸虫的感染导致山羊细胞免疫呈抑制状态 ;

In this study,abilities of proliferation and secreting IL 2 and NK activity of peripheral blood lymphocytes (PBLs) of goats and buffaloes experimentally infectes with Fasciola hepatica were detected by indirect methods.The results showed that proliferation of goats in two groups(each acute infected with 200 or 500 metacercarines) decreased significantly(P<0.05,or P<0.01).While proliferation of water buffaloes(each chronically infected with 1200 metacercariaes)were significantly higher than those of controls(P<0.05).NK activity of uninfected buffaloes were higher than that of goats.NK activity of infected goats had two tops,while there was no significant change of NK activity of infected buffaloes.IL 2 of uninfected buffaloes were higher than that of goats.IL 2 of both infected animals increased significaltly (P<0.05,of P<0.01),while proliferation of infected PBLs decreased stimulated by the recombined IL 2 of cattle (r IL 2).This indicated that infestation of Fasciola hepatica made animals a cellular immune disorder and there is some mechanism to prevent IL 2 from protective action in TH 1 group.In a word,water buffaloes were more resistant than goats to the infestation of Fasciola hepatica because the infection made cellular immune of goats restrained,while infected water buffaloes kept quite high cellular immune level.