网状内皮组织增殖病病毒(REV)不同分离株LTR基因的序列分析

Sequence analysis of Long terminal repeats(LTR) gene of REV between different isolated strains

从山东、江苏的几个大型鸡场收集疑为REV感染的鸡脾脏、肝脏 ,提取组织DNA ,并以此为模板 ,通过PCR技术 ,从山东泰安及江苏海安的某鸡场中分别扩增到REV的LTR基因。将该基因克隆进TA载体中 ,转化大肠杆菌TG1,经酶切鉴定 ,得到了两株REVLTR基因的克隆 ,标记为SD和HA。将所得克隆测序并与国外报道的REVLTR基因进行比较 ,发现国内分离株与国外株有

Two strains of Reticuloendotheliosis (REV) were amplified by polymerase Chain Reaction (PCR) from field samples.The amplified gene for long terminal repeats(LTR) of REV was cloned into TA vector.The recombinant plasmids marked SD and HA were studied in detail by restriction endonuclease analysis and nucleotide sequencing.The sequences were compared with the reported LTR gene of REV A strain,and the results show a high homology (over 94%).