番茄愈伤组织和植株耐盐性的比较研究

Comparative studies on the reaction to NaCl treatment of callus and plant from tomato

对 2份野生种和 4份栽培种的研究证明 ,子叶、下胚轴和叶片外植体诱导的愈伤组织对NaCl的反应是一致的。将4份野生种和 2份栽培种带有 2叶一心的小苗扦插于附加不同浓度NaCl的MS培养基中 ,发现在 15 0mmolNaCl处理 10d时 ,1号、2号、3号和 4号材料的相对生根率分别为 6 5 .8% ,87.5 % ,86 .8%和 10 0 % ;4份野生种在附加有 75mmolNaCl的培养基上的生长优于对照 ,随着NaCl浓度的不断提高 ,株高和叶片数都表现下降 ,3号材料下降幅度最小。当NaCl浓度提高到 30 0mmol时 ,4份野生种的鲜重可达对照的 0 .90倍、1.0 7倍、1.6 0倍和 1.5 3倍 ,而 2份栽培种则为对照的 0 .5 5倍和0 .

The similar reaction of the calli from cotyledon,hypocotyl and leaf blade explants to NaCl treatment was observed. The possibility of using in vitro shoot culture to evaluate salt tolerance of different tomato genotypes was determined. Shoot with 2 leaves were grown on media supplemented with 0, 75, 150, 300, 450mmol NaCl,and rooting and growth were determined. This different degree of salt tolerance among species was clearly shown on the basis of the callus growth, shoot rooting and growth. The results showed that Lycopersicon pennellii LA716 was the most salt tolerant among the wild species. In vitro culture could be a better system for testing and selecting for salt tolerance.