毫米波联合放射对人舌鳞癌细胞生长的抑制效应

Effects of Millimeter Wave Combined with γ ray Radiation on Human Tongue Squamous Cell Carcinoma Cell

目的 观察毫米波联合放射对Tca8113细胞集落形成及超微结构的影响。方法 采用高低功率密度毫米波联合60 Coγ射线辐射人舌鳞癌Tca8113细胞悬液 ,3周后观察细胞集落形成抑制率 ,2 4h后行电镜检查。结果 各实验组细胞集落形成率均有显著下降 (P <0 .0 0 1) ;集落形成抑制率高功率密度组高于低功率密度组 (P <0 .0 5或P<0 .0 1) ;长照射时间组高于短时间照射组 (P <0 .0 5 ) ;先后放疗组无显著差异 (p >0 .0 5 ) ;联合组高于单纯毫米波照射组和单纯放射组 (P <0 .0 0 1或P <0 .0 5 )。扫描及透射电镜下分别见细胞表面损伤性改变 ,内部结构退行性变化。高功率密度组改变程度较低功率密度组严重。结论 毫米波辐射能明显抑制Tca8113细胞的集落形成能力 。

Objective To observe the effects of millimeter wave combined with 60 Co γ ray radiation on human tongue squamous cell carcinoma cell(Tca8113). Methods Using mm wave combined with 60 Co γ ray to irradiate Tca8113 cell suspensions. The colony forming inhibition efficiency was observed three weeks later. 24 hours after radiation, the samples were observed under electron microscope. Results The study showed that the radiation could result in significant decrease of the colony forming efficiency ( P< 0.001). The inhibition efficiency was higher when the samples were exposed to high power density mm wave or for a long duration ( P< 0.05 or P< 0.01). The inhibiting effect in combined groups was more obvious than in singly radiated group ( P< 0.001 or P< 0.05). There was no difference between ‘R+HL’ group and ‘HL+R’ group ( P >0.05). Electron microscopy showed the cells' suprastructures had some injuries and regressive changes. And more serious changes existed in ‘H’ group.Conclusion It could be concluded that mm wave radiation could efficiently inhibit the colony forming capability of Tca8113 cell. And mm wave radiation could lead to morphological changes of exterior or interior ultrastructures of Tca8113 cell. [