补充铁、锌对染锰小鼠毒性的影响

The Effect of Prophylactic Supplementation of Iron and Zinc on the Toxicity of Manganese in Mice

[目的 ]观察补充铁、锌对染锰小鼠的神经行为、精子变化、脑组织神经细胞超微结构的影响。[方法 ]对染锰小鼠同步补充铁、锌后 ,进行旷场行为测试、精子形态观察、脑组织细胞电镜的观察。 [结果 ]单染毒组与低剂量补铁、锌组相比 ,小鼠出现探索潜伏期时间缩短 (P <0 0 5 ) ;精子总数增加、精子畸形率降低 (P <0 0 5 ) ;脑组织细胞电镜观察中发现低剂量组预防效果为最好 ,其次为中剂量组 ,而高剂量组则无效果。 [结论 ]提示补充铁、锌对染锰小鼠的神经行为、小脑神经细胞的形态结构及精子变化均具有一定的预防效应 ,其改善程度与铁。

Objective] To examine the effects of iron and zinc prophylactic supplementation on the toxicity of manganese in mice in three aspects;(1)neurobehavioral function,(2)sperm shape and function,(3)brain of neuron ultrastructure.To determine what level of zinc and iron supplementation is the most effective in perventing toxic effect of manganese.[Method]After ip administering a dose of 55 mg/kg of manganese chloride,then the mice were divided into four groups,one is control group,other three groups were supplied different amount of zinc and iron through the drinking water.An open field test,sperm analysis and electronic microscopy of brain tissue were carried out. [Results] It was found that only the low iron and zinc supplemented group showed significantly reduction of toxic effect of manganese based on the three aspects tested.Those groups given high doses of iron and zinc did not show a significant effect on manganese toxicity. [Conclusion] Iron and zinc can be successfully used as an antidote to manganese toxic effects,but only at low dose.