肝炎患者肝组织表皮生长因子及其受体表达的研究

Expression of EGF and EGF R in Liver Tissues From Patients with Viral Hepatitis

目的 研究各型肝炎患者肝组织内表皮生长因子 (EGF)及其受体 (EGF R)表达的变化 ,以进一步探讨EGF用于治疗重型肝炎的可能性。方法 采用免疫组织化学技术 (ABC法 )观察 6 5例各型肝炎患者、4例健康成人、1例胎肝组织内EGF/EGF R的表达情况。结果 ① 4例正常成人肝组织EGF R皆为阳性 ,且愈近中央静脉染色愈深 ;②肝炎患者肝组织EGF染色与正常成人肝组织相似 ,未发现显著改变。③患者肝组织中EGF R的表达与坏死程度有关 ,随着坏死程度的加重 ,坏死区内染色增强。结论 提示给予外源性EGF可能进一步促进肝组织的再生 。

Objective To study the expression of EGF and EGF R in liver tissues from patients with viral hepatitis. Methods Expression of EGF and its receptor(EGF R) was identified on frozen sections from liver tissues of 65 patients,4 normal adults and human fetus by avidin biotin immunoperoxidase complex technique. Results ①Positive staining of EGF R was present widely in liver tissues from all normal adults except fetus.The expression of EGF R increased in necrotic regions and decreased in non necrotic regions in all patients;②positive staining of EGF only presented in epithelium cells of bile duct in liver tissues from normal adults but was stronger in some hepatocytes from fetus.The staining was similar in the patients and normal adults;③these changes were closely related to the degree of liver necrosis. Conclusion The EGF/EGF R system may play an important role in liver regeneration and promote certain beneficial effect on the prognosis of patients with severe viral hepatitis.EGF administration in vivo may be used to increase survival rate.