抗生素S_(632)A_3在体外抗肠道病毒及单纯疱疹病毒I型(HSV-1)作用的初步研究

Inhibitory effects of antibiotic S_(632)A_3 on enterovirus and HSV-1 in vitro

目的 :体外测定抗生素S632A3 抗肠道病毒及单纯疱疹病毒I型的作用 ,为筛选新型抗病毒药物提供参考依据。方法 :用不同稀释度的抗生素S632A3 抑制3种肠道病毒及单纯疱疹病毒I型对Vero细胞的感染作用 ,48h后观察细胞病变效应 ,并用MTT法测定细胞活性。结果 :抗生素S632A3 能明显抑制肠道病毒及疱疹病毒对Vero细胞的致病变作用 ,使细胞存活率升高。结论 :该药物有较显著的抗肠道病毒及单纯疱疹病毒I型的作用 ,且对细胞的毒性很低 ,是一种具有潜在开发前景的药物。

Objective: To assay the inhibitory effects of antibiotic S 632A 3 on enterovirus and herpes simplex type-1 virus(HSV-1) in vitro in order to select a new kind of antiviral drug. Methods: Vero cells were infected by different enteroviruses and were cultured with serial dilutions of S 632A 3.The inhibitory effects of S 632A 3 were evaluated with cell survival rates by MTT method and CPE.Results: The cytopathogenic effects (CPE)of cells infected by enterovirus or HSV-1 were inhibited significantly, resulting in higher cell survival rates. Conclusion:The results have shown that S 632A 3 had obviously enterovirus-inhibitory efficacy and low cytotoxity,therefore,it may become a potential candidate in drug development and deserves further research on animal experiments and antiviral mechanisms.