Bio-Normalizer(BN)对体外鼠胚大脑神经细胞缺氧损伤保护作用的研究

Protective effect of Bio-Normalizer (BN) on cerebral neurocytes in rat fetus following anoxic damage in vitro

目的 :为研究Bio -Normalizer(BN)对大脑神经细胞缺氧损伤的保护作用 ,并探讨其机制。方法 :本研究采用无血清体外细胞培养方法 ,培养鼠胚大脑神经细胞 ,将神经细胞造成缺氧损伤 ,在无血清培养液中加入不同剂量的BN ,观察BN对缺氧损伤的保护作用。将细胞分为4组 ,Ⅰ组为缺氧BN浓度为0mg/ml组 ;Ⅱ组为缺氧BN浓度为0.1mg/ml组 ;Ⅲ组为缺氧BN浓度为0.5mg/ml组 ;Ⅳ组为非缺氧BN浓度为0mg/ml组。在倒置显微镜下观察细胞生长发育的情况 ,于细胞培养后的第4天收集细胞 ,测定生化指标 ,同时进行光镜和电镜病理检查。结果 :缺氧0.1mgBN/ml组的神经细胞MTT及NSE活性均高于缺氧0mgBN/ml组 ,并有显著性差别 (P<0.05) ;形态学观察结果也显示缺氧0.1mgBN/ml组和0.5mgBN/ml组的神经细胞缺氧损伤后恢复明显好于缺氧0mgBN/ml组。结论 :BN对大脑神经细胞缺氧损伤恢复具有明显的促进作用。

Objective: To explore the protective effect of Bio-Normalizer (BN, papaya) on rat embryonic cerebral neurocytes in vitro. Methods: The rat embryonic cerebral neurocytes were divided into four groups ( I group anoxic damage 0mgBN/ml,Ⅱ group anoxic damage 0.1mgBN/ml,Ⅲ group anoxic damage 0.5mgBN/ml and Ⅳgroup non-anoxic damage 0mgBN/ml). The growth, development and morphology of neurocytes were observed by light and electronic microscopes. The activity of neuron- specific enolase (NSE) and MTT of neurocytes were detected. Results:The activity of NSE and MTT of neurocytes in anoxic damage 0.1mgBN/ml group were significantly higher than that in anoxic damage 0mgBN/ml in vitro (P<0.05). The morphological observation showed that the recovery of anoxic damage in anoxic damage 0.1mgBN/ml and anoxic damage 0.5mgBN/ml groups was significantly better than that in anoxic damage 0mgBN/ml group in vitro. Conclusions: The Bio-Normalizer (BN) can promote the recovery of anoxic damage of rat embryonic cerebral neurocytes in vitro.