大黄素对内毒素刺激下的大鼠腹腔巨噬细胞分泌TNF_α及NO的影响

The Influence of Emodin on the Production of TNF_α and Nitric Oxide by Peritoneal Macrophages of Rats

目的 :研究大黄素抗炎作用机理。方法:利用脂多糖 (Lipopolysacchride ,LPS)刺激的大鼠腹腔巨噬细胞(PMΦ)作为人体过度炎症反应的体外模型 ,用MTT法和荧光法测定了大黄素对不同状态下的巨噬细胞分泌TN Fα、NO的影响。结果:大黄素可通过抑制LPS刺激的大鼠腹腔巨噬细胞分泌TNFα ,抑制过度的炎症反应 ;而对于未经LPS刺激的大鼠腹腔巨噬细胞 ,大黄素可促进TNFα的分泌。大黄素能抑制炎症反应中的NO的大量合成与释放。结论:大黄素对机体的免疫功能可能具有双向的调节作用。

Objective: To study the possible antiinflammatory mechanism of emodin(EMD). Methods:The effects of emodin were studied on the secretion of TNFα and the synthesis and release of nitric oxide from macrophages under different conditions with MTT method and fluorometry. Results:The results showed that emodin could inhibit severe inflammation by inhibiting the secretion of TNFα and decreasing the level of nitric oxide in macrophages previously stimulated with LPS. For the macrophages with no LPS-stimulation, EMD could promote the secretion of TNFα.Conclusion: EMD might have the ability of up/down immunomodulation.