牛磺酸对丝裂霉素C诱发人淋巴细胞遗传损伤的保护作用

The protective effect of taurine on genetic damage in human lymphocytes induced by mitomycin C

目的 :研究牛磺酸的抗诱变作用。方法 :人体外周血淋巴细胞分别加入0.5～20.0mmol/L牛磺酸及0.05mg/L丝裂霉素C(MMC) ,(37±1)℃体外培养72h ,观察淋巴细胞姊妹染色单体互换 (SCE)频率和染色体畸变(CA)发生率。结果 :0.5～5.0mmol/L牛磺酸加0.05mg/LMMC组淋巴细胞SCE及CA发生率与阳性对照组(MMC0.05mg/L)相近 (P>0.05) ;而当牛磺酸浓度≥10.0mmol/L时 ,淋巴细胞SCE及CA频率明显降低 (P<0.05) ,并呈现浓度依赖趋势。结论 :较高浓度 (10mmol/L及以上 )牛磺酸可以对化学诱变剂所致DNA和染色体的遗传损伤具有一定保护作用。牛磺酸很可能是一种有前景的化学诱变保护剂。

Objective: To investigate the antimutagenicity of taurine.Methods : Cells from human peripheral blood were cultured in vitro in the presence of 0.5 to 20.0 mmol/L taurine and 0.05mg/L mitomycin C (MMC) for 72h at 37±1℃. Lymphocytes were examined for frequency of sister chromatid exchange (SCE) and for incidence of chromosome aberration (CA). Results :The frequencies of SCE and CA were similar in the lymphocytes exposed to 0.5 to 5.0 mmol/L taurine plus 0.05mg/L MMC and positive controls (0.05mg/L MMC). However the lymphocytes which were cultured in the presence of ≥10.0 mmol/L concentrations of taurine exhibited a statistical significant decrease in the frequencies of SCE and CA, and a concentration-dependent trend.Conclusion: At higher concentrations( ≥10.0 mmol/L ), taurine exhibited a protective effect on chemical mutagen-induced genetic damage of DNA and chromosome.Taurine could be a promising chemoprotective agent against mutagenesis.