双色荧光原位杂交检测苯系物暴露工人精子9、18号染色体数目畸变

Detection of Numerical Chromosome Aberrations in Sperm of Workers Exposed to Benzene Series by Two-color Fluorescence in situ Hybridization

为研究苯系物暴露工人精子常染色体数目畸变。用地高辛标记的９号染色体探针（Ｄ９Ｚ１）和生物素标记的１８号染色体探针（Ｄ１８Ｚ１）进行双色荧光原位杂交，测定精子９、１８号染色体非整倍体率。车间空气苯时间加权平均浓度（ＴＷＡ）为８６．４９ｍｇ／ｍ３，高于国家卫生标准１倍，苯暴露工人尿粘糠酸（ｔｔＭＡ）显著高于对照组。共计数１４名暴露工人１３６ ４０１条精子，１６名对照工人１５６ ９５５条精子。暴露组９、１８染色体双体精子率（分别为０．１６８％± ０．０６３％、０．０５５％± ０．０３１％）和二倍体精子率（０．０７３％± ０．０４５％）均高于对照组相应数值（分别为０．０５０％±０．０３０％、０．０３３％±０．０２５％和０．０４０％±０．０３６％）；暴露组９、１８号染色体缺体率（分别为０．２０６％±０．０４７％，０．０６８％±０．０４４％）高于对照组值（０．０６７％±０．０３７％、０．０４８％±０．０３４％）。总数目畸变率（０．５７０％±０．１４４％）亦高于对照组值（０．２１８％±０．０７１％）。实验表明接触较高浓度苯可引起长期暴露者精子常染色体非整倍体率增高。

To study the frequency of numerical chromosome aberrations in sperm of workers exposed to benzene series, the aneuploidy frequencies of 9 and 18 chromosomes were detected in sperm of workers by two color fluorescence in situ hybridization with digoxingenin labeled 9 chromosome probe (D9zl) and biotin labelled 18 chromosome probe (D18zl). The timeweight average air concentration (TWA) of benzene in the workplace was 86.49mg/m3, it was two fold higher than the national maximum allowable concentration. The concentration of urinary trans, transmuconic acid (ttMA) in exposed group was significantly higher than that of control group. The sperms counted in 14 exposed workers and 16 control workers were 136 401 and 156 955 respectively. The results showed that the disomy frequencies of sperm for chromosome 9 and 18 (0.168% ±0.063% and 0.055% ±0.031% respectively) and the frequency of diploidy sperm (0.073% ±0.045%) were statistically increased over that of the control group (0.050% ±0.030%; 0.033% ±0.025% and 0.040% ±0.036%, respectively). The nullisomic frequencies of 9 and 18 chromosome in the exposed group (0.206% ±0.047%, 0.068% ±0.044%) were statistically increased over that of the control group too (0.067% ±0.037%, 0.048% ±0.034%). The frequency of overall numerical chromosome aberrations in the exposed group was 0.570% ±0.144%, when this value was compared with that of control (0.218% ±0.071%), a statistically significance was presented. Our experiments showed that exposed to benzene at higher concentration may induce increase in aneuploidy frequency of sperm autosomal chromosome in exposed workers.