摘要
通过有性杂交手段培育出聚合有不同数目Ｂｔ基因的植株，在不同生育期进行抗虫性测定和Ｂｔ毒蛋白表达的ＥＬＩＳＡ检测，旨在揭示聚合不同数目Ｂｔ基因的植株抗虫性的互作表达机理．聚合有１—４个Ｂｔ基因的植株在整个生育期的抗虫性、毒蛋白表达特性和单价抗虫棉时空表达一致，生育前期抗虫性好，毒蛋白表达量高；生育中、后期抗虫性有所下降，毒蛋白表达量降低。聚合有４个Ｂｔ基因的纯合材料并未因Ｂｔ基因的增加而起到抗性增强的效果，相反还因同源抑制而有所降低．不同来源的Ｂｔ基因处于杂合状态时其抗虫性和Ｂｔ毒蛋白量均得到充分表达。
The plants with various dosages of inserted Bt genes were developed by intercrossing among transgenic Bt lines in which Bt genes were inserted in different chromosomes, and commercial non-transgenic Bt varieties. Laboratory bioassay of insect-resistance and Bt toxin protein assays with ELISA were used to detect efficacy to bollworm in different cotton growing stages. As transgenic Bt insect-resistant cotton, temporal difference of resistance existed in plants converging on different dosages of inserted Bt genes, i.e., there was a declining level of efficacy with plant age as the mortality (%) of Helicoverpa and Bt toxin protein expressional level decreased gradually, when analyzed with leaves from the main stem. Our research also revealed that the insect-resistance of converging 4 Bt genes plants was decreased due to homozygous suppression.
基金
国家"863"高科技研究项目
关键词
转BT基因棉花
聚合
抗虫性
育种利用
剂量
transgenic Bt gene cotton
convergence
insect-resistance
breeding exploitation
