用光声光谱技术测量鼻咽癌细胞的光谱吸收特性

PHOTOACOUSTIC ABSORPTION CHARACTERISTICS OF SUNE-1, CNE-2,AND CNE-1 HUMAN NASOPHARYNGEAL CARCINOMA CELL LINESMEASURED USING PHOTOACOUSTIC TECHNIQUES

为了解人鼻咽癌细胞的光谱吸收特性,用双光束归一化光声光谱技术测量三株人鼻咽癌细胞 (SUNE -1 ,CNE -2 ,CNE -1)的归一化吸收光谱和光谱吸收系数。结果表明 :(1)在波长4000～6000 范围内 ,这三株人鼻咽癌细胞的光谱吸收系数大小为 :SUNE -1>CNE -2>CNE -1 ;(2)在波长4200 处平均相对光声信号值为 :SUNE -1 :1.36 ,CNE -2 :1.20,CNE -1 :1.10 ,三者之间的差异具有显著性 (P值=0.012)。人鼻咽癌细胞株SUNE -1 ,CNE -2和CNE -1具有不同的光声光谱特性。

Purpose: To investigate optical absorption characteristics of human nasopharyngeal carcinoma cell lines. Methods: Standardized photoacoustic spectroscopy and optical absorption coefficient of SUNE-1,CNE-2,and CNE-1 were measured respectively using double beam standardized photoacoustic technique. Results:(1)in the range from 4000? to 6000?,optical absorption coefficient of three cell lines are in the following order: SUNE-1>CNE-2>CNE-1;(2)At wavelength 4200?, the mean values of relative photoacoustic signal for SUNE-1, CNE-2, and CNE-1 are 1.36, 1.20,and 1.10 respectively, showing a singnificant difference(p=0.012). Conclusion: Human nasopharyngeal carcinoma cell lines SUNE-1, CNE-2, and CNE-1 have different photoacoustic spectroscopic characteristics. This study lays a foundation of further research on biological behaviors of nasopharyngeal carcinoma.