龙牙楤木幼芽体细胞胚胎发生和植株再生(简报)

SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN VITRO FROM YOUNG SHOOTS OF ARALIA ELATE (MIQ.) SEEM.

龙牙楤木[Aralia elate(Miq.)Seem]是五加科楤木属的多年生乔本药用植物,又称刺老鸦,具有补气、活血、祛风、利湿、止痛等功效。主要分布于我国东北地区、朝鲜、日本和俄罗斯的西伯利亚地区。其幼嫩茎叶是有名的山菜,在我国及东南亚一些国家很受欢迎。但是,近些年来由于人为恶性采伐。

Explants excised from the young shoots of Aralia elata (Miq. ) Seem, were cultured on MS media. Calli were induced from the explants on MS medium supplemented with 0. 5mg/L 2, 4-D, 0. 5mg/L BA and 0. 5mg/L NAA. Then these calli were transferred onto the MS medium containing 2. 0mg/L 2, 4-D + 0. 5mg/L BA + 0.5mg/L NAA and 0.2% activated charcoal. Under these conditions the somatic embryoids were observed and regenerated plants were obtained from somatic embryoge-nesis. Then, a experimental system with stability and high regenerating efficiency has been set up for the propagation of the young plants, the cell breeding technology and the control of somatic embryogenesis of Aralia elata (Miq.) .