栽培稻与疣粒野生稻杂种F_1代的基因组原位杂交鉴定

Identification of Hybrid of Oryza sativa×O.meyeriana Using Genomic in situ Hybridization

生物素标记的疣粒野生稻总 DNA作探针 ,未标记的栽培稻总 DNA封阻 ,对栽培稻与疣粒野生稻杂种 F1体细胞染色体进行基因组原位杂交 (Genomic in situ hybridization,简称 GISH)分析。 FITC检测表明 ,杂种细胞中来自疣粒野生稻的染色体有较多的黄色或黄绿色荧光信号 ,来自栽培稻的染色体只检出很少的信号。每条疣粒野生稻染色体上信号点所占的总的区域只是染色体的一小部分 ,表明疣粒野生稻染色体与栽培稻染色体的

With biotin labelled total genomic DNAs from Chinese wild rice, Oryza meyeriana as probe and 4 fold unlabelled total genomic DNA from cultivated rice,O.sativa IR36 as blocking,genomic in situ hybridization was applied to somatic chromosome preparations of O.sativa×O.meyeriana F 1 hybrid.When detected with anti biotin FITC,O.meyeriana chromosomes gave gree nish yellow fluorescence while cultivated rice chromosomes fluoresced red with PI counterstain.The area of signal on each wild chromosome was small.It suggested that a great part of DNA sequence in O.meyeriana is homologous to cultivated rice.