海南粗榧(Cephalotaxus mannii Hk.f.)5个种群遗传多样性及分化研究

Genetic Diversity and Population Differentiation of Cephalotaxus mannii Hk.f.Populations

采用垂直板型聚丙烯酰胺凝胶电泳测定了海南粗榧 5个种群遗传多样性和遗传分化程度。结果表明 :海南粗榧种群遗传多样性水平较低 ,多态位点比率 P=0 .33,等位基因平均数 A=1 .33,平均期望杂合度为 H e=0 .1 35,观察杂合度为 H o=0 .1 39。种群间遗传分化程度较低 ,基因分化系数 Gst=0 .1 2 3,种群间遗传一致度和遗传距离的均值分别为 0 .971 9、0 .0 2 88。黎母山种群与其它种群分化最大 。

Genetic diversity and population differentiation among 5 Cepha lotaxus mannii Hk.f.populations were investigated using the assay of vertical slab polyacrylamide gel electrophoresis.Cephalotaxus mannii Hk.f.maintained relatively low level of genetic variation with P =0 33, A =1 33, He =0 135 and Ho =0 139.Genetic differentiation ( Gst ) was low among populations, Gst was 0 123,and the means of genetic identity and genetic distance were 0 9719 and 0 0288 respectively.The greatest differentiation between Mt.Limu population and others may be attributable to the prevalent wind direction in the season of pollination.