若干理化因子对日本肾蕨绿色球状体分化及生长的影响

THE EFFECTS OF PHYSICAL AND CHEMICAL FACTORS ON DIFFERENTIATION AND GROWTH OF GREEN GLOBULAR BODIES OF NEPHROLEPIS CORDIFOLIA

通过对日本肾蕨（Ｎｅｐｈｒｏｌｅｐｉｓ ｃｏｒｄｉｆｏｌｉａ）绿色球状体（ＧＧＢ）的培养实验，研究了不同物理、化学因子对其分化及生长的影响。结果表明，辐照度１００ μｍｏｌ ｍ～（－２）Ｓ～（－１）对ＧＧＢ的分化及生长均有明显促进作用．红光照射对ＧＧＢ的分化比白光和蓝光的好。此外，ＧＧＢ的分化以 ＭＳ＋ ６－ＢＡ １ ｍｇ Ｌ～（－１）＋ＮａＨ２ＰＯ４ ２００ ｍｇ Ｌ～（－１）为好。

Green globular bodies (GGB) first obtained from runner tips of Nephrolepis cordifolia Presl were cultured on MS medium supplemented with 4 mg L-1 BA and 0.1 mg L-1 IAA at 23 ℃ under continuous fluorescent light at 40 μmol m-2s-1 for 30 days. These GGB were then subcultured on the same medium every month. The differentiation and the growth of GGB subcultured on varies media or with different growth regulators, and various light quality and light intensity were examined and calculated after 70 days. The results showed that stimulating effects on the differentiation and growth were observed at irradiance of 100 μmol m-2s-1.Red light was better for differentiation of GGB than blue or white light. In addition, MS medium supplemented with 1 mg L-1 6-BA and 200 mg L-1 NaH2PO4 was best for differentiation of GGB.