摘要
通过对日本肾蕨(Nephrolepis cordifolia)绿色球状体(GGB)的培养实验,研究了不同物理、化学因子对其分化及生长的影响。结果表明,辐照度100 μmol m~(-2)S~(-1)对GGB的分化及生长均有明显促进作用.红光照射对GGB的分化比白光和蓝光的好。此外,GGB的分化以 MS+ 6-BA 1 mg L~(-1)+NaH2PO4 200 mg L~(-1)为好。
Green globular bodies (GGB) first obtained from runner tips of Nephrolepis cordifolia Presl were cultured on MS medium supplemented with 4 mg L-1 BA and 0.1 mg L-1 IAA at 23 ℃ under continuous fluorescent light at 40 μmol m-2s-1 for 30 days. These GGB were then subcultured on the same medium every month. The differentiation and the growth of GGB subcultured on varies media or with different growth regulators, and various light quality and light intensity were examined and calculated after 70 days. The results showed that stimulating effects on the differentiation and growth were observed at irradiance of 100 μmol m-2s-1.Red light was better for differentiation of GGB than blue or white light. In addition, MS medium supplemented with 1 mg L-1 6-BA and 200 mg L-1 NaH2PO4 was best for differentiation of GGB.
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2001年第2期142-148,共7页
Journal of Tropical and Subtropical Botany
关键词
日本肾蕨
绿色球状体
分化
生长
理化因子
Nephrolepis cordifolia
Green globular body
Differentiation
Growth