冰核微生物中冰核基因重复序列PCR分析

PCR OF THE MAIN REPETITION SEQUENCE OF THE INAZ GENE IN THE DNA OF INA^- AND INA^+ BACTERIA AND FUNGI

在本试验的条件下 ,PseudomonoassvringaeinaZ基因产生的蛋白的主要重复基元的编码区 :5’ GCCGGTTATGGCAGCACGCTGACC 3’序列既在冰核真菌、细菌中存在 ,也在非冰核真菌、细菌中存在 ,即冰核细菌、真菌和非冰核细菌、真菌都有扩增产物 ,并且产物呈多态性 ,同一个种不同菌株间也呈多态性 ,说明该引物不适合用于鉴定真菌、细菌中冰核基因是否存在 ,也不能用于区分冰核真菌和非冰核真菌以及区分冰核细菌和非冰核细菌 ,更不能根据其扩增片段的量和大小说明冰核真菌、细菌冰核活性的强弱。

The oligonucleotide primer having the 5’\|GCCGGTTATGGC AGCACGCTGACC\|3’ was synthesized,which encodes a main repetition motif of protein produced by the inaZ gene.PCR of the primer in the DNA of INA - and INA + bacteria and fungi was done.All of the DNA of INA - and INA + bacteria and fungi expressed bands,the results show that the primer did not work for distinguishing the INA - and INA + organisms and for identification of the ice nucleation activity of the INA organisms by the size and brightness of the PCR products.