过碘酸盐氧化法制备固相载体除抗非目的蛋白抗体

REMOVING UNSPECIFIC ANTIBODIES WITH SEPHACRYL GEL COUPLING OF E COLI PROTEIN BY PERIODATE OXIDATION

Sephacryl凝胶经过碘酸盐氧化将产生醛基 ,非目的蛋白通过氨基与醛基反应结合到凝胶上制备固相载体。装柱后 ,抗体经过长时间循环上样 ,抗非目的蛋白的抗体被吸收 ,抗体得到了纯化。用经本法处理过的抗体做免疫印迹 。

The total proteins of plain E.coli(DH5 α)were coupled to the Sephacryl S300 oxidized with periodate,and the preliminarily purified antiserum was loaded repeatedly on the column for 18 hours.The unspecific anti\|E.coli antibodies would be absorbed and the antibodies would be purified.It was more specific to do western blot using antibodies treated by this method than using ones without treatment.