EB病毒潜伏膜蛋白1调控细胞凋亡的cDNA阵列分析

Study of Apoptosis Regulated by Epstein-Barr Virus Encoded Latent Membrane Protein 1 Using Apoptosis cDNA Expression Array

为探讨EB病毒潜伏膜蛋白 (LMP1)介导细胞凋亡和抑制细胞凋亡双重效应的机制 ,采用已建立的受四环素调控的LMP1表达的鼻咽癌细胞系 (pTet on LMP1HNE2 ) ,定量诱导 pTet on LMP1HNE2细胞LMP1动态表达 ,分别与含有细胞凋亡相关基因为主的AtlasapoptosiscDNAexpressionarray膜杂交 ,分析LMP1介导的表达差异基因。结果表明 :①LMP1介导细胞凋亡和抑制细胞凋亡的基因的表达 ,同时上调或下调某些与细胞凋亡相关基因的表达 ;②LMP1不仅介导细胞凋亡和抑制凋亡基因的表达 ,同时介导细胞分裂分化和增殖基因的表达 ,LMP1同时介导功能不同甚至功能相反的基因表达 ;③LMP1介导的基因表达与其表达持续时间和表达水平相关 ,不同表达水平和不同表达时间介导的基因表达谱不同。因此 ,LMP1具有介导细胞凋亡和抑制凋亡基因表达的双重生物学效应 ,同时介导细胞增殖、细胞周期、细胞凋亡等多种生物学效应基因的表达 ,从而参与细胞的癌变。

To probe the mechanism of apoptosis and anti apoptosis regulated by Epstein Barr virus latent membrane protein(LMP1),Doxycycline(Dox)induced LMP1 expression in pTet on LMP1 HNE2 cells at high and low levels at different times were analyzed by Western blot.To identify LMP1 regulated gene expression,total RNAs were respectively extracted from these cell groups that were treated with Dox of 6μg/ml,0.06μg/ml for 12h and 24h respectively.Reverse transcripted probes from the total RNAs were respectively hybridized to the Atlas apoptosis cDNA expression array.The results showed that LMP1 up regulated the expression of some genes related to apoptosis and anti apoptosis,and down regulated the expression of some other genes of apoptosis and anti apoptosis;LMP1 regulated the expression of the genes of apoptosis,proliferation and cell division either in a positive or in a negative manner at the same time;LMP1 affected the expression of different gene sets on different expression levels at different times.These may suggest that LMP1 can trigger pleiotropic roles of apoptosis and anti apoptosis;LMP1 is involved in carcinogenesis via its regulating the expression of genes which are associated with cell cycle,proliferation and apoptosis.