银杏木质部特异定位表达基因启动子克隆

Cloning of xylem-specific-expression promoter of glycine-rich-protein (GRP 1.8) gene from Ginkgo biloba.

用PCR方法成功地从木本植物银杏基因组总DNA中扩增克隆得到木质部细胞特异性表达的富甘氨酸蛋白质基因 (GRP1.8)的启动子 ,并克隆到pUC18载体中 .与菜豆的序列比较发现 ,碱基同源性高达 98%以上 .除发现启动子特有的TATAbox序列外 。

Xylem\|specific\|expression promoter of the glycine\|rich\|protein (GRP1.8) gene was amplified by PCR method from total DNA extracted from \%Ginkgo biloba,\% and cloning into pUC18 vector. The PCR products was sequenced. Compared with the sequence of French bean, extremely high cogenetic was found about 98%. The specific sequence is GATAG.