飞秒激光探测PSⅡ高效传能途径和技术研究

Study on Route and Technology of PSⅡ High Efficiency Energy Transfer Using Laser Detection

采用可独立调谐的单色、双色以及三色飞秒激光光谱学、能量传递动力学和瞬态荧光动力学 ,研究了PSⅡ能量传递及转化的微观机理 .结合国内外电子结晶PSⅡ二维结构和三维结构图 ,确定了激发能从LHCⅡ ,通过CP2 9、CP2 6、CP2 4和CP47、CP43分别传递到达反应中心的途径 .对独立调谐的双色、三色飞秒激光探测技术进行了探索性研究 ,结果表明 ,利用多激发态光谱学、选择性超快时间分辨率光谱学和程控飞秒光化学反应动力学可有效进行光合作用微观机理的超快过程研究 .

By using independent tuning single color, two color or even three color femtosecond laser spectroscopy, energy transfer dynamics and transient fluorescence dynamics, the micromechanism of PSⅡ energy transfer and transform was studied. The route of excitation energy transfer from LHCⅡ to reaction center through CP29, CP26, CP24 and CP43, CP47 has been determined with the help of the two dimensional and three dimensional structures of PSⅡ by electron crystallography. Recent experiments on independent tuning two color and three color femtosecond laser detection technology indicated that study of the ultrafast process of photosynthesis micromechanism using multi excited state spectroscopy, selectivity ultrafast time resolved spectroscopy and process control femtosecond photochemistry reaction dynamics can be carried out effectively.