甲真菌病患者免疫功能的测定与分析

Test and analysis of immunity function in patient with onychomycosis

目的 探讨甲真菌病患者免疫学方面的异常。方法 采用单克隆抗体间接免疫荧光法测定了 5 0例甲真菌病患者外周血T淋巴细胞亚群水平以及采用双抗体夹心ELISA法测定患者血清IL 2、sIL 2R的水平。结果 甲真菌病患者外周血T+8细胞数增加、T+3 细胞数减少 ,与正常对照组相比差异有显著性 (P <0 0 1)。T+4 细胞数、IL 2、sIL 2R水平与正常对照组相比较变化不明显 (P >0 0 5 )。

Objective To research the immunology variance of onychomycosis patients. Methods we used APAAP for the quantitative determination of T lymphocyte sub populations and used double antibody sandwich ELISA for testing the levels of IL 2 and sIL 2R in the peripheral blood of 50 patients with onychomycosis. Results The number of T + 8 cell increased; the number of T + 3 cell reduced; they were apparendly different from those of the controls ( P <0 01). The number of T + 4 cell, the levels of IL 2 and sIL 2R were not apparendly different from those in healthy controls( P >0 05). Conclusion Dysfunction of cellular immunity has some effect on pathogenesis of onychomycosis.