微血管计数、CD_(44)V_6表达与乳腺癌临床病理因素及预后的关系

Prognostic Significance of Microvessel Count and Expression of CD_(44)V_6 in Invasive Breast Carcinoma

目的：探讨乳腺癌组织中微血管计数、ＣＤ４４Ｖ６表达与临床病理因素及预后的关系。方法：对经治疗后随访８年以上的乳腺癌病例，应用免疫组化法，分别检测癌组织中微血管计数及ＣＤ４４Ｖ６表达。将其结果与影响预后的临床病理因素及生存状况比较。结果：肿瘤内微血管数与组织学分级、临床分期、淋巴结转移呈正相关。死亡组癌组织内微血管数明显高于存活组。 ＣＤ４４Ｖ６在癌组织中的表达率为 ８０．４％，淋巴结阳性及组织学分级高的病例 ＣＤ４４Ｖ６阳性表达率增加，死亡组ＣＤ４４Ｖ６表达明显高于生存组。结论：微血管计数及ＣＤ４４Ｖ６可做为估计乳腺癌预后和指导临床治疗有价值的参考指标。

Objective: To study the relationship between microvessel count, the expression of CD44V6 and the prognosis in invasive breast carcinoma. Methods: This study comprised 31 patients with axillary node negative breast carcinoma and 25 patients with positive axillary lymph nodes who had a follow up period of 8 years. Microvessels were identified by immunohistochemistry using antibody to factor Ⅷ-related,antigen. Three most vascular areas within the tumor were selected, and the microvessels within a 200 times microscopic field of each area were counted by two investigators. The level of CD44V6 (56 cases) expression was also observed. Then compared the results with clinical and pathological factors which affected the prognosis. Statistical significance of difference between the dead patients and the survivals was made. Results: The microvessels quantitation of the deaths was much higher than that of the survivals (P<0.01). Microvessel counts had positive correlation with lymph node metastasis and histologic grade. Positive rate of CD44V6 expression was 80.4% and it increased in node positive patients and in patients who hadhigher histologic grade. The CD44V6 expression was also significantly increased in dead patients than that of the survivals. Conclusion: Microvessel quantitation may be a useful predictor for identifying breast cancer patients at high risk for relapse and death. UP regulation of CD44V6 expression correlates with badly biological behavior of breast cancer. Extensive therapy should be necessary for such patients.