维甲酸对人舌鳞癌细胞株Tca83 Fas/FasL基因表达的影响

Alteration of Fas/FasL gene expression in oral squamous cell carcinoma cell line Tca83 by retinoid acid

目的 探讨不同浓度全反式维甲酸 (all transretinoidacid ,简称RA) ,在不同作用时间下对人舌鳞癌细胞株Tca83诱导凋亡过程中 ,相关凋亡基因Fas/FasL的表达及两者之间可能的关系。方法 采用TUNEL法检测RA作用下细胞发生凋亡的情况 ;应用RT PCR和细胞免疫组化方法检测药物作用组和对照组细胞Fas蛋白水平和mRNA水平的表达。结果 TUNEL法显示生理药物浓度 1 10uRA作用到第 5d时可见明显的凋亡细胞出现 ,与对照组相比差异有显著性 (P <0 0 5 )。相应的RT PCR亦显示RA作用到 5d时FasmRNA水平明显提高 ,而细胞的FasLmRNA水平却没有改变。细胞免疫组化结果也显示RA可增强Fas抗原的表达。结论 RA能诱导Tca83的凋亡基因Fas的表达。提示临床上可利用RA作为先期诱导药或与增强Fas表达的药物、抗Fas抗体联合应用 ,以增强RA药效 ,降低不良反应。

Objective To study the induction of apoptosis by retinoid acid(RA) in Tca83 cell line and the expression of correlative Fas/FasL gene. Methods Apoptosis of Tca83 was detected by TUNEL, the expression of Fas/FasL mRNA was detected by RT PCR and cell immunohistochemical technique. Results The cell apoptosis number of Tca83 in RA group was significantly higher than that in non RA groupe on day 5( P< 0 05). The exepression of Fas mRNA in RA group was also much higher than that in non RA group on day 5 ( P< 0 05), but the level of FasLmRNR had no change. Immunohistochemistry also showed, after RA treatment, the Fas stain was enhanced. Conclusion The result suggests that apoptosis induced by RA may be resulted from the enhancement of Fas gene transcription and translation.