腺病毒介导的p16和p53的联合应用对胆管癌细胞QBC939的生长抑制作用

INHIBITORY EFFECTS OF HUMAN CHOLANGIOCARCINOMA CELL LINE BY RECOMBINANT ADENOVIRUSES p16 AND p53 TRANSDUCTION

为研究p16和 p5 3基因的联合应用对胆管癌细胞的作用 ,将重组体腺病毒 p16、p5 3、p16联合 p5 3转移到人胆管癌细胞QBC939,对 p16、p5 3基因的表达、细胞的生长抑制及机制进行了分析。RT PCR显示在胆管癌QBC939细胞系中p16呈低表达 ,p5 3不表达。重组体腺病毒能介导 p16和p5 3等外源基因在胆管癌QBC939细胞系中高效表达 ,两者联合应用能明显抑制QBC939细胞的生长和集落形成。流式细胞计数证实其能诱导QBC939细胞发生明显凋亡并导致其发生G1期阻滞。本研究显示 p16及 p5 3基因通过诱导肿瘤细胞凋亡及G1期阻滞在肿瘤的基因治疗方面发挥作用 。

To evaluate the inhibitory effect of recombinant adenoviruses p16 (Ad p16) combined with Ad p53 on human cholangiocarcinoma cell line QBC939 in vitro. Expression of p16 was low and p53 was negative in QBC939 cells. High level of p16 and p53 expressions were observed in QBC939 cell line transfected with Ad p16 and Ad p53. The growth rates of the Ad p16 infected combined with Ad p53 infected QBC939 cells were inhibited, which was higher than those of the Ad p53 or close to Ad p16 transfection. Colony formation in Ad p16 with Ad p53 transfected cells greatly decreased versus Ad p16 transfected or Ad p53 transfected cells. The Ad p16, Ad p53 and Ad p16 with Ad p53 transfected cells manifested apoptosis and G 1 arrest, which were confirmed by the flow cytometry. The suppression effects mediated by expression of the exogenous p16 with p53 in tumor cell resulted mainly from apoptosis and G 1 arrest, which is higher than exogenous p16 or p53.