MG_7Ag在胃癌细胞系SGC7901及其耐药亚系中的表达及功能

EXPRESSION AND FUNCTION OF MG_7 Ag ON GASTRIC CANCER CELL LINE SGC 7901 AND ITS DRUG RESISTANT SUBLINE SGC 7901/VCR

探讨MG_7Ag在胃癌药敏细胞系SGC 790 1及其耐药亚系SGC 790 1/VCR 0 3、SGC 790 1/VCR 0 7、SGC 790 1/VCR 1 0中的表达及功能。采用免疫细胞化学方法及流式细胞仪观察MG7Ag在胃癌药敏细胞及其耐药细胞系的表达 ;用MTT法检测单克隆抗体MG7对药物敏感性的影响 ;以阿霉素作为荧光标记用流式细胞仪观察MG7对细胞内药物浓度的影响。结果表明 ,MG7Ag在耐药细胞系的表达较药敏细胞明显增加 ,且随耐药指数的增加呈逐渐增加的趋势 ;胃癌药敏细胞及其耐药细胞与MG7共同孵育后 ,对长春新碱的药物敏感性减低 ;MG7与耐药细胞共同孵育后 ,细胞内阿霉素的浓度减低。MG7Ag可能作为一个新的与胃癌耐药相关的分子 ,在维持胃癌耐药细胞系SGC 790

To explore the expression and function of MG 7 Ag on gastric cancer cell line SGC7901 and its drug resistant subline,SGC7901/VCR, with different resistance index (0 3, 0 7, 1 0) The expression of MG 7 Ag on SGC7901 and SGC7901/VCR cells was determined by immunohistochemical method and flow cytometry (FCM) MTT assay was used to explore the effects of monoclonal antibody MG 7 on drug sensitivity of gastric cancer cells FCM was used to explore the effects of MG 7 on adriamicine accumulation of SGC7901/VCR cells SGC7901/VCR cells exhibit increasing positive staining of MG 7 than SGC7901, according to the increasing resistance index Sensitivity of vincristine of both SGC7901 and SGC7901/VCR cells was reduced after being treated with MG 7 And SGC7901/VCR cells showed decreased adriamicine accumulation when they were incubated with MG 7 Therefore, the conclusion is that MG 7 Ag may be a novel molecule associated with multidrug resistance of gastric cancer, and play an important role in maintaining the drug resistant phenotype of SGC7901/VCR cells.