心肌毛细血管六胺银染色法及缺血预适应对心肌毛细血管的影响

A SPECIFIC STAINING METHOD OF MYOCARDIAL CAPILLARIES AND THE EFFECT OF ISCHEMIC PRECONDITIONING ON MYOCARDIAL CAPILLARIES

探索显示心肌毛细血管的方法及用该法观察缺血预适应 (ischemicpreconditioning ,IP)对毛细血管的影响。制备套扎前降支动脉犬模型 ,缺血再灌注 (ischemia reperfusion ,IR)组予缺血 1h ,再灌注 2h ;IP组在缺血与再灌注之前 ,予缺血和再灌注各 5min ,反复 4次。实验终点取缺血心肌进行六胺银染色显示毛细血管 ,并定量比较。结果六胺银法染色显示心肌毛细血管清晰 ;IP组缺血心肌毛细血管数、周长和面积密度较IR组增加。认为六胺银法显示心肌毛细血管可靠实用 ;IP减轻缺血后毛细血管的破坏 。

To explore a method of identifying myocardial capillaries and to use it to observe the effects of ischemic preconditioning on the myocardial capillaries. Anesthetized open chest dogs in ischemic preconditioning (IP) group ( n =6) were subjected to 5min ischemia and 5min reperfusion for 4 cycles before 1 hour of left descending coronary artery (LDA) occlusion followed by 2 hours of reperfusion, while in the ischemia reperfusion (IR) group ( n =6) they were only subjected to 1 hour occlusion followed by 2 hour reperfusion. Myocardial samples in ischemic area were obtained to display capillaries by silver hexamethylenetetramine staining, and their quantitative analysis was conducted by imaging analyzing system. Two growps were then compared. The results showed that myocardial capillaries were clearly shown by silver hexamethylenetetramine, and it revealed that the numerical points density, numerical perimeter density and numerical profile density of myocardial capillaries in ischemic region were obviously increased in IP group compared with IR group. The results suggested the method to identify myocardial capillaries by silver hexamethylenetetramine was reliable and practical, and IP could protect the myocardial capillaries from the damage of ischemia reperfusion.