TTV在恒河猴实验感染的初步研究

STUDY OF TT VIRUS IN EXPERIMENTALLY INFECTED RHESUS MONKEY

通过TTV在恒河猴的实验感染 ,了解TTV的嗜肝性及其在各组织中的分布。从 5只实验感染病毒的恒河猴各组织中抽提DNA ,经PCR检测各组织中TTV的感染 ;分别用病毒双链探针或单链负义探针 ,与吸附在纤维膜上的组织DNA进行斑点杂交。PCR结果与双链探针的杂交结果一致 ,肝、骨髓、脾、胃、小肠和大肠组织及血清均阳性 ,都有TTV的感染。单链负义探针与各组织DNA杂交 ,仅在肝、骨髓和小肠为阳性 ,表示肝、骨髓和小肠可能有作为病毒复制中间体的正义DNA。提示TTV能侵染恒河猴的许多组织 ,但仅在肝、骨髓和小肠可能是病毒的复制场所 。

To study tissue distribution of TTV in experimentally infected Rhesus monkey and if the TT virus is hepatotropic. Total DNA was extracted from tissues of 5 experimentally infected Rhesus monkeys. TTV was detected by PCR,and dot hybridization was done with virus double DNA strand probe or single antisense strand probe. The double strand probe was hybridized with DNA of liver, bone marrow, and spleen, stomach, small intestine, colon. and sera. In the above tissues , the virus was also positive as shown by PCR. The single strand antisense probe was only hybridized with DNA of liver, bone marrow,and small intestine of all 5 monkeys, but not with that of other tissues. It suggested that TTV could infect many tissues of Rhesus monkey. Only the liver ,bone marrow, and small intestine presented the virus positive single strand, which might be a replicative intermediate of the virus. It suggests that TT virus replicates in liver , bone marrow, and small intestine,and it might be hepatotropic.