肝素对刀豆蛋白A诱导昆明小鼠急性肝损伤的保护作用

THE PROTECTIVE EFFECT OF HEPARIN ON ConA INDUCED ACUTE LIVER INJURY IN KUNMING MICE

为了观察肝素预处理对刀豆蛋白A(ConA)诱导昆明小鼠急性肝损伤的保护作用 ,将 2 4只昆明小鼠随机均分为A、B、C 3组。A组为正常对照组 ;B组经小鼠尾静脉一次性注射给予一定剂量的ConA ,造成急性重型肝损伤 ;C组在给予ConA同时皮下注射肝素 10 0U。结果发现 ,与单纯ConA处理组相比 ,肝素预处理组小鼠 8h死亡率明显降低 ,血清ALT水平下降 (P <0 .0 5 ) ,肝窦瘀血及肝脏炎症程度明显减轻 ,肝组织匀浆中MDA水平显著降低。因此认为 。

To study the effect of heparin on the ConA induced acute liver injury inKunming mice,twenty four mice were randomly divided into groups A,B and C.To the mice in group A, 0 2ml of normal saline (NS) was intravenously administered, while those in group B were given ConA 18mg/kg instead of NS in order to induce severe acute liver injury. Heparin was injected subcutaneously in a dose of 100U per animal at the same time as ConA challenge in group C. Compared with group B, prior injection of heparin in group C significantly decreased the mice death rate and the peak levels of serum ALT within 8h. At the same time, intrasinusoidal congestion and hepatic inflammation were alleviated significantly,and MDA level in liver homogenate was lowered markedly. It suggested that heparin is efficient in protecting the mice from liver injury induced by ConA.