应用cDNA芯片分析79个新基因的人胚组织表达谱

The 79 Novel Genes Expression Pattern in Human Fetal Tissues was Monitored by Using cDNA Microarray

大规模cDNA测序和生物信息学技术相结合 ,得到来自于商品化的人胚肾cDNA文库 79个代表新基因的表达序列标签 (EST) .随后 ,采用高速度机械手制备这些cDNA的基因芯片 ,用于鉴定 79个新基因的ESTs在2 0周、 2 6周两个胚胎时期 6种组织中的基因表达状况 ,以研究这些EST片段代表的新基因功能提供线索 .通过芯片杂交及结果分析 ,得到同一个组织两个不同时相 8个差异表达的基因 。

79 EST fragments which represents corresponding novel genes were obtained by sequencing and bioinformatics analysis of human fetal kidney cDNA library. Microarray was prepared by using these novel EST fragments by automatic spotting. Expression patters of 79 ESTs of novel genes from human fetal kidney were analyzed in fetal brain and fetal heart tissues of 20-week-age and 26-week-age fetus by performing of cDNA chip hybridization. This provides clues for studying exact functions of the novel genes. 8 genes were obtained which were expressed differentially in the fetal brain and heart of 20-week-age and 26-week-age respectively. Then differentially expressed genes were identified by Northern analysis. The more exact function of the novel genes are under study.