微水相中杏仁醇腈酶催化不对称合成(R)-氰醇的研究

Study on the Asymmetric Synthesis of (R)-Cyanohydrins Catalysed by (R)-Oxynitrilase from Almond in Micro-aqueous Phase

利用气相色谱手性分析 ,研究了微水相中来源于杏仁的 (R) 醇腈酶催化醛与HCN不对称合成 (R) 氰醇 .结果表明 ,反应时间、添加乙酸、反应介质、反应体系水活度、反应温度和底物的结构对醇腈酶反应均有显著影响 .杏仁醇腈酶对芳香族、脂肪族和杂环族醛均有良好的催化作用 .其中 ,苯甲醛为杏仁醇腈酶的最适作用底物 ,在低温 (0～ 5℃ )下 ,转化率和产物对映体过剩值均在 99%以上 .

Asymmetric synthesis of (R) -cyanohydrins from aldehydes and hydrogen cyanide using (R) -oxynitrilase from almond in micro-aqueous phase was studied by GC chiral analysis. Reaction time, content of acetic acid, reaction medium, water activity, reaction temperature and substrate structure all had remarkable effects on the reaction. (R) -oxynitrilase from almond accommodates a wide variety of aromatic, heterocyclic, and aliphatic aldehydes. Benzaldehyde was found to be the best substrate for the (R) -oxynitrilase from almond. At low temperature 0 similar to5 degreesC, both conversion rate and enantiomeric excess of the product were above 99%.