核磁共振研究羟苯氨酮对离体灌流心脏能量代谢的影响

Effects of Oxyphenamone on Energy Metabolism of Perfused Rat Hearts Exposed to Ischemia Characterized by ^(31)P NMR

羟苯氨酮是一种新型强心扩血管剂 ,在以往的研究中发现 ,羟苯氨酮对实验性心衰和心肌缺血再灌注损伤有保护作用 .利用3 1P核磁共振表面线圈技术 ,对羟苯氨酮在Langendorff模型的离体灌流心脏 6 0min缺血 6 0min再灌注中的作用机制进行了探讨 .实验结果显示给药组胞内pH值比对照组恢复得更快 ,但是胞内高能磷酸化合物的含量却低于对照组 .羟苯氨酮对缺血 再灌注损伤的保护作用表现在提高心肌细胞的能量代谢水平 。

The effect of oxyphenamone (Oxy, a new inodilator) on high energy phosphate metabolism of isolated Langendorff perfused rat hearts exposed to 60 minutes of ischemia and 60 minutes of reperfusion injury was determined using phosphorus nuclear magnetic resonance ( 31 P NMR) spectroscopy. The results led to a paradox of rapid intracellular pH recovery and a low level of high energy phosphate pools reserve during reperfusion compared to the control. It can be stated that Oxy improves energy consumption and, in turn, improves pH recovery, but also causes a deletion of high energy phosphate pools during reperfusion. The relationship between ions regulation, including H + regulation, and myocardial functions recovery may display a key factor for cell survival during postischemic reperfusion.