摘要
NGX6是克隆的鼻咽癌相关基因 ,它的功能与作用机制目前尚不十分清楚 .通过脂质体转染把NGX6导入鼻咽癌细胞株中 ,采用双向凝胶电泳分离细胞内所有蛋白质 ,通过软件分析 ,找到与未处理细胞表达差异的蛋白质 ,通过质谱分析和生物信息学资料处理 .鉴定出七种表达上调的蛋白质 ,其中包括Fas蛋白 ,锌指蛋白(ZNF) ,主要组织相容性抗原Ⅱ (MHCⅡ )等 .Fas蛋白参与细胞凋亡的信号传导途径 ,它的上调可以促进细胞凋亡 ;ZNF蛋白参与基因的转录调控 ,它的上调也可影响细胞异常增殖的信号传导通路 ;MHCⅡ可以促进机体对肿瘤细胞的免疫应答 .这些结果说明NGX6可能通过多种途径抑制鼻咽癌细胞的生长 ,为研究NGX6的作用机制提供了很好的实验资料 ,对鼻咽癌的基因治疗奠定了一定的研究基础 。
The current study was designed to reveal the preliminary function of NGX6 to NPC cells. In search of mechanisms of NGX6 2 D PAGE was used to identify proteins that were overexpressed in HNE cells that were transfected with NGX6. After staining and image analysis, spots of interest were isolated and subjected to mass spectrometry. Seven proteins such as Fas,ZNF and MHC ⅡAg were identified. The possible significance of these findings is discussed. NGX6 may exert its effect on NPC cells by many ways.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2001年第4期573-578,共6页
Progress In Biochemistry and Biophysics
基金
国家"8 63"高科技发展计划 (10 2 10 0 1 0 5 )
国家自然科学基金资助项目 (3 990 0 163和 3 0 0 0 0 96)~~
关键词
双向电泳
细胞转染
基质辅助性激光解吸飞行时间质谱
鼻咽癌相关基因
two dimensional gel electrophoresis,cell transfection,matrix assisted laser desorption,ionization mass spectrometry