斑腿蝗科11种蝗虫RAPD带型的变异

COMPARISON OF RAPD PATTERNS VARIATIONS IN 11 SPECIES OF CATANTOPIDAE

采用随机扩增多态性DNA (RAPD)技术研究了斑腿蝗科 8属 11种蝗虫RAPD带型的变异。在 2 3个随机引物中有 8个引物能扩增出清晰而稳定的RAPD带 ,扩增总带数为 148条。带型显示 :科内属间及属内种间多态性普遍存在。利用Nei&Li相似系数和UPGMA聚类法 ,构建斑腿蝗科不同属种的分子系统树。聚类结果显示 :属内的种各自先聚为一类 ,而 8个属间的系统树分为两大分支 ,这两个分支与这些属种的形态特征尤其是翅发达与否完全一致。第一分支的 2个属 (云秃蝗属和小蹦蝗属 )均为前翅退化型 ,第二分支的 6个属 (星翅蝗属、长夹蝗属、素木蝗属、胸斑蝗属、外斑腿蝗属、斜翅蝗属 )都是前翅发达型。

Catantopidae is a dominant family of Acridodea and has about over 3 000 speices. In order to investigate genetic relationships,random amplified polymorphic DNA(RAPD) technique was applied to assess RAPD patterns variations in 11 species belonging to 8 genera of Catantopidae. Among 23 arbitrary primers(10 bp)under predetermined optimal reaction conditions,8 primers were informative and yielded a total of 148 clear and reproducible bands. The patterns showed polymorphic variations among and within different genera and species. RAPD data were used to calculate Nei's similarity index. Molecular phylogentic tree were constructed by unweighted pair-group method with arithmetical averages (UPGMA). The results showed the species of each genus first clustered respectively and 8 genera of Catantopidae were divided into two branches, whose conclusion coincided with morpholgical (eg. wingmorphs). In one branch, 2 genera (Yunnanacris Chang and Pedopodisma Zheng)had short fore-wings, whereas 6 genera (Calliptamus Serville, Choroedocus Ⅰ. Bolivar, Shirakiacris Dirsh, Apalacris Walker, Xenocatantops Dirsh and Eucoptacra Ⅰ. Bolivar)in the other branch had long fore-wings. From these results, we found that the species with similar morphological characters shared a higher similarity on RAPD bands.