福建九龙江口红树植物分子分类的研究

MOLECULAR PHYLOGENY OF SEVEN SPECIES OF MANGROVES IN JIULONG RIVER IN FUJIAN

运用RAPD技术对福建九龙江口龙海红树林自然保护区浮宫种苗园内白骨壤(Avicenniamarina)、桐花树(Aegicerascorniculatum)、无瓣海桑(Sonneratiaapetala)、秋茄(Kandeliacandel)、木榄(Bruguieragymnorrhiza)、海莲(B.sexangula)、尖瓣海莲(B.sexangulavar.rhyn chopetala)等7种红树植物进行了遗传多样性分析。从30个10 mer随机引物中筛选出15个有效引物 ,利用这15个有效引物共扩增出630条DNA带 ,其中多态性条带535条 ,占总扩增条带的84.92 %。利用Nei指数法得出7种红树植物间的遗传一致度和遗传距离 ,并运用UPGA统计分析法对红树植物的7个种间的亲缘关系进行聚类分析。7个种分为A、B两个大组。白骨壤、桐花树、无瓣海桑同属于A组。秋茄、木榄、海莲、尖瓣海莲分子聚类在B组。分子聚类结果和传统的分类学相吻合 ,由此表明这15个有效引物的RAPD分子标记技术能较为客观地反应出红树植物种间的遗传亲缘关系 ,并为从分子水平研究红树植物遗传多样性、保护。

DNAs from 7 species of mangroves were extracted by the CTAB method. A 260/A 280 value of DNA solutions ranged from 1.6 to 2.0 and the length of DNA fragment was about 23 kb. RAPD analysises on genetic diversity of mangroves were conducted. 15 effective primers were screened from 30 10 oligonucleotide arbitrary primers, and a total of 630 DNA bands were amplified, among which 535 (84.92%) were polymorphic. Based on UPGA cluster analysis on DNA bands amplified by the 15 primers, a DNA molecular dendrogram was established. A standard delimit of genetic distance in mangroves was also established, and compared to the conventional classification, the molecular classification of mangroves was accurate. All results from the analyses of molecular phylogeny could be valuable to the projects of plant introduction, ecological restoration and conservation in mangroves.