癌基因ras对β-1,4-半乳糖基转移酶活性的调节

The Regulation of Activity of β-1,4-Galactosyltransferase by Oncogene ras

研究癌基因ras对细胞表面的 β 1,4 半乳糖基转移酶活性的调节 构建Ha ras表达载体并转染NIH 3T3细胞株 ,测定细胞表面和细胞内 β 1,4 半乳糖基转移酶活性和其mRNA的水平 结果发现ras使NIH 3T3细胞表面的 β 1,4 半乳糖基转移酶活性降低 ,而高尔基体内的活性不变 此外用Northern印迹检测后发现 ,ras不能改变细胞内 β 1,4 半乳糖基转移酶的mRNA水平 这说明癌基因ras能够调节细胞表面β 1,4 半乳糖基转移酶活性 。

In order to investigate the regulation of β 1,4 galactosyltransferase (GalTase) activity by oncogene Ha ras , expression vector of oncogene Ha ras was constructed and transfected into NIH 3T3 cell line. The activities of cell surface and total GalTase activity were assayed by HPLC method. The mRNA of GalTase activity was also evaluated by Northern blot. It was found that the activity of cell surface GalTase was decreased by ras transfection and the enzyme activity of Golgi apparatus remained constant. The mRNA level of GalTase does not be changed by ras . The results showed that the activities of GalTase might be regulated by Ras signal pathway.