回转器模拟微重力刺激对不同品系稻田鱼腥藻代谢特性的影响

EFFECT OF MICROGRAVITY SIMULATION WITH CLINOSTAT ON DIFFERENT STRAINS IN ANABEANA ORYZA

以稻田鱼腥藻空间搭载克隆株 (AoSR1 6)、回复再搭载克隆株 (AoSR1 6 1 7)和原始出发株 (AnabeanaoryzaHB2 3)为材料 ,通过回转器模拟微重力刺激实验 ,对不同品系稻田鱼腥藻的微重力生物学效应进行了分析。结果发现 ,模拟微重力刺激对稻田鱼腥藻不同品系均表现出一定的生长刺激效应 ,尤为空间飞行后的克隆株更为明显。比较三个品系在微重力刺激下的光合与呼吸活性 ,原始出发株的光合与呼吸活性明显高于空间搭载株。在回转器培养情况下 ,具有高固氮酶活性的克隆株 (AoSR1 6和AoSR1 6 1 7)所固定的氮 ,除了用于藻细胞正常的生命活动外 ,主要用于其生长增强效应 ,而藻胆蛋白累积量和氨分泌量较之对照培养时要少得多。对回转器培养后的稻田鱼腥藻进行单克隆分离 ,结果没有出现类似于空间搭载实验的性状分离现象。

The Biological responses of Anabana oryza (different strains) to microgravity simulation with clinostat were analyzed.The simulation of growth rate was observed under clinoration all the strains,especially in flight strains (AoR16 and AoSR16-17). Anabana oryza HB23 showed higher activity than flight strains in photosynthesis and respiration.The nitrogen which flight strains with high nitrogrnase activity fixed under culturing in ground was used to accumulate phycobiliprotein in cell and excrete ammonium out of cell after meeting the normal metabolism,however,it was mainly used to simulate growth rate under culturing with clinostat.menwhile,it was different from space flight that microgravity simulation with clinostat was not able to induced the phenotype segregation in Anabana oryzae.