大豆叶片C_4循环途径酶

C_4 Pathway Enzymes in Soybean Leaves

以不同产量水平的大豆 (Glycinemax (L .)Merr.)品种“黑农 40”和“黑农 37”为实验材料 ,研究了苗期、开花期、初荚期和鼓粒期等不同发育时期的叶片中磷酸烯醇式丙酮酸羧化酶 (PEPCase)、NADP_苹果酸脱氢酶 (NADP_MDH)、NADP_苹果酸酶 (NADP_ME)、丙酮酸磷酸双激酶 (PPDK)等C4途径的主要酶和 1 ,5_二磷酸核酮糖羧化酶(RuBPCase)的活性变化。结果表明两种大豆叶片均含有上述 4种酶 ,而且从PEPCase/RuBPCase的比值看 ,C4酶在高产大豆“黑农 40”叶片中表达较高。表明C4循环途径与大豆产量密切相关。这一结果还暗示有可能通过检测C4途径酶的表达水平及比例 ,筛选出具有高产潜力的大豆种质。

High-yielding soybean (Glycine max (L.) Merr.) variety, 'Heinong 40', and one control variety, 'Heinong 37', were used as experimental materials. The activities Of C-4 pathway enzymes, i.e. PEPCase (phosphoenolpyruvate carboxylase), NADP-ME (NADP-malic enzyme), NADP-MDH (NADP-malate dehydrogenase), PPDK (pyruvate phosphate dikinase) and RuBPCase (ribulose-1.5-bisphosphate carbozylase) were assayed at seedling, blooming, pod-bearing and pod-filling stage. The results indicated that C4 pathway enzymes were expressed differently at different developing stages in both varieties of soybean, but the ratio of PEPCase and RuBPCase showed that the expression of C4 pathway enzymes of 'Heinong 40' was higher than those of 'Heinong 37' at each stage. The results showed that C4 pathway enzymes were closely related to the crop yielding potential. Therefore, it is possible to select potentially high-yielding soybean variety by the expression Of C4 pathway enzymes.