摘要
The storage glutelin in rice ( Oryza sativa L.) seeds could be separated at least into more than 13 acidic and 19 basic polypeptides by two dimensional electrophoresis. Rice glutelin might be mainly controlled by about six major genes according to the expression of glutelin polypeptides. The acidic polypeptide of glutelin could be clearly separated into two groups by peptide mapping and N-terminal amino acid sequence analysis. These two groups were just in accord with the GluA and GluB subfamilies exactly.
利用灵敏的等点聚焦与SDS_PAGE结合的双向电泳分析方法 ,从水稻 (OryzasativaL .)种子贮藏谷蛋白中至少可以分离为 1 3条酸性和 1 9条碱性多肽。依据谷蛋白多肽的表现量推测 ,水稻谷蛋白主要由约 6个主效基因控制。肽图谱与N_端氨基酸序列分析可清晰将谷蛋白酸性多肽分为两组。此两组恰好与谷蛋白GluA和GluB两个cDNA克隆组相吻合。
