用于监测环境中酪氨酸酶抑制剂的植物组织电极

A PLANT TISSUE BIOELECTRODE FOR ENVIRONMENTALMONITORING OF TYROSINASE INHIBITOR

根据二乙基二硫代氨基甲酸盐、硫脲或苯甲酸等环境污染物质对蘑菇富含的酪氨酸酶所产生的抑制作用,研制了将蘑菇固定在碳糊而成的植物组织电极,用来有效地监测上述酪氨酸酶抑制剂．当存在酶的底物儿茶酚时,在该蘑菇组织电极上通过批量加入以及流动注射两种实验方式进行电流分析测定,结果表明这种生物传感器价廉、灵敏、快速、操作简易(无须温育)等,可用于现场环境监测 另外,还对申极制作条件诸如蘑菇用量及蘑菇切片部位等因素对电极行为的影响进行了研究．

The inhibition of tyrosinase contained in mushroom by diethyldithiocarbamate, thiourea, or benzoic acid was exploited for effective monitoring of these toxin pollutants. In view of the above, plant tissue bioelectrodes based on immobilization of mushroom within carbon psate were developed. Amperometric measurements at these biosensors, carried out in the presence of the catechol sub-strate, were illustrated either in the batch addition or in flow injection experimntes. The results show that the mushroom-carbon paste tissue electrode offered many advantages such as cheapness, high sensitivity and speed, and no need for incubation period, thus being led to on-line applica-tions for monitoring of the environment. Furthermore, the effects of the mushroom loading and sec-tion on inhibition responses were explored to be conductive to a better knowledge of possible sensing mechanisms at tissue-carbon paste bioelectrodes.