克雷伯氏菌微氧发酵生产1,3-丙二醇的研究

Study on microaerobic conversion of glycerin to 1,3-propanediol by Klebsielle pneumoniae

对克雷伯氏菌在厌氧和通 1 .2L/min空气的条件下间歇连续发酵生产 1 ,3 丙二醇进行了研究 ,在两种条件下 1 ,3 丙二醇的转化率接近。在通空气的条件下细胞能够正常代谢 ,并获得较高菌体密度 ,但有氧发酵的生产强度高于厌氧发酵 ;甘油处于限制状态、稀释率为 0 .1h- 1 的连续发酵中 ,有氧发酵 1 ,3 丙二醇的转化率和生产强度均高于厌氧发酵。

The conversion of glycerin to 1,3-propanediol (1,3-PD) by Klebsielle pneumoniae with batch and continuous fermentation processes under anaerobic and microaerobic conditions was studied. The 1,3-PD conversion rates of both processes approached to the same level, but the productivity of 1,3-PD under microaerobic condition was higher than that under anaerobic condition inasmuch as normal metabolism of cells. In the continuous culture at a dilution rate of 0.1 h-1 and glycerin limitation, both yield and productivity of 1,3-PD under microaerobic condition were higher than that under anaerobic condition.