胃癌组织中细胞凋亡及PCNA表达的意义

Significance of Apoptosis and Proliferating Cell Nuclear Antigen Expression in Gastric Carcinoma

[目的]研究细胞凋亡、增殖细胞核抗原(PCNA)表达与胃癌临床病理因素及预后之间关系。[方法]采用脱氧核苷酸末端标记法(TUNEL法)及免疫组化SP法分别对110例胃癌组织的细胞凋亡指数(AI)及PCNA表达进行检测 ,分析细胞凋亡指数(AI)及PCNA表达与胃癌临床病理及预后因素之间的关系。[结果]细胞凋亡指数(AI)在分化型胃癌明显高于未分化型癌(P<0 01) ,未侵及浆膜无淋巴结转移胃癌的AI明显高于浆膜受累及有淋巴结转移者(P均<0 01)。PCNA表达阳性率在未分化型癌明显高于分化型癌(P<0 005) ,且浸润浆膜并有淋巴结转移癌的PCNA表达阳性率明显高于未侵及浆膜 ,无淋巴结转移者(P均<0 005)。生存期≥5年胃癌者的AI明显高于生存期<5年者 ,而PCNA阳性表达率则明显低于生存期<5年者。PCNA阳性表达率与细胞凋亡指数之间呈负相关。[结论]细胞凋亡和PCNA表达对胃癌的生物学行为及预后评定具有一定的意义。PCNA与细胞凋亡呈负相关 ,联合检测两者对评估胃癌发展及预后有协同作用。

To investigate the correlation between apoptosis proliferating cell nuclear antigen (PCNA) expression and clinicopathologic factors prognosis in gastric carcinoma (GC).Cell apoptosis index (AI) and PCNA expression of 110 specimens from patients with GC were investigated by TUNEL technic and SP immunohistochemical staining method.Correlations between AI,PCNA expression and clinicopathologic factors,prognosis were analyzed.AI of differentiated GC was significantly higher than that of undifferentiated GC (P<0 01),and AI was also significantly higher in GC patients without serosal invasion,lymph node metastasis than that in those with invasion or metastasis (P<0 01).The incidence of PCNA expression in undifferentiated GC was significantly higher than that of differentiated GC (P<0 005),and PCNA positivity was significantly higher in GC patients with serosal invasion,lymph node metastasis than that in those without invasion or metastasis (P<0 005).AI of tumor with survival ≥ 5 years was significantly higher than that with surival < 5 years,and PCNA positivity of tumor with survival ≥5 years was significantly lower than that with survival < 5 years.There was a negative relationship between PCNA positivity and AI.[Conclusions]Apoptosis and PCNA expression are significant for estimating the biological behavior and prognosis in GC.Combined determination plays a synergic efficacy.