胸部开放伤后海水浸泡对实验犬肾脏病理形态学的影响

Effect of open chest injury and seawater immersion on pathological changes in the kidney

目的 :探讨胸部开放伤后海水浸泡对实验犬肾脏病理形态学的影响。方法 :实验动物致伤后随机分为对照组 (n=5 ) ;单纯胸伤组 (n=10 ,致伤后直接观察 4小时 ) ;海水浸泡组 (n=10 ,致伤后立即放入人工配制的海水中浸泡 4小时 )。采用光镜和电镜对肾组织进行病理形态学检查 ,并于致伤前及伤后 (浸泡后 ) 15、30、45分钟以及 1、2、3和 4小时采取血标本 ,采用酶联免疫吸附 (EL ISA)法测定血浆肿瘤坏死因子α(TNFα)和白介素 1β(IL 1β)水平。结果 :光镜检查表现为部分肾小球毛细血管丛体积缩小 ,腔内空虚、塌陷 ,部分扩张充血 ,体积增大。电镜下可见足细胞内线粒体肿胀 ,内质网扩张 ,足突变形、融合 ;肾小管上皮浊肿 ,部分有透明变性及空泡变性 ,部分有斑片状坏死 ,并可见远曲小管上皮消失 ,管腔内有蛋白管型 ,肾间质血管充血。海水浸泡组伤后血浆 TNFα和 IL 1β表达明显升高 ,且高峰出现时间明显提前。结论

Objective:To evaluate the effect of open chest injury and seawater immersion on pathological changes in the kidney.Methods:Twentyfive healthy dogs were divided into three group:control group ( n =5),simple chest injury group ( n =10) and seawater immersion group ( n =10).A model of right open pneumothorax was established.Animals were immersed in artificial seawater after chest injury in seawater immersion group.Blood samples were taken at eight different time intervals to determine serum tumor necrosis factor (TNFα)and interleukin1β (IL1β).Pathological examination of the kidney was studied by light microscope and electronic microscope.Results:The pathological examination showed various signs of renal injury.The plasma levels of TNFα and IL1β significantly increased at 30 minutes and peaked at 60 minutes after seawater immersion.The time of peak level appeared earlier in seawater immersion group than that in control group.Conclusions:The pathological changes in the kidney appears to be associated with seawater immersion after chest trauma.