心康口服液预防小鼠病毒性心肌炎的实验研究

Experimental study of preventive effect of Xin-Kang oral liquid on axcute viral myocarditis in mice

目的 探讨心康口服液预防小鼠柯萨奇B3病毒性心肌炎的作用。方法 心康口服液预防性用药 2d后的小鼠经腹腔感染CVB3 ,以诱发急性病毒性心肌炎模型 ,持续用药至 2 0d ,观察小鼠于感染不同阶段的心肌组织形态学改变并进行形态定量分析 ,所得数据用SPSS PC软件包作统计学处理。结果 心康口服液各剂量组的心脏表面重度病变检出率显著低于病毒对照组 (P <0 0 1) ;心肌组织重度损伤 (心肌细胞大片坏死崩解 )的检出率均低于病毒组及干扰素组 ,其中中剂量组与病毒组差异显著 (P <0 0 1) ;心肌平均病变面积、病变面积 全心面积比值第 2 0d时心康组与干扰素组均与病毒组差异显著 (P <0 0 1) ,第 5d时心康各剂量组与病毒组差异显著 (P <0 0 1)。结论 心康口服液预防性给药可保护心肌 ,减轻心肌损伤及促进损伤心肌恢复 ,其预防柯萨奇B3病毒性心肌炎的作用类似干扰素。

Objective To study the preventive effect of Xin\|Kang oral liquid on acute Coxsackie B3 viral myocarditis in mice.Methods The mice were infected Coxsackie virus B3 (CVB3m) through abdominal cavity after they were fed with the Xin\|Kang oral liquid for 2d, and the uses of the drug continued to the 20th day. Histopathological changes of the myocardial cells were observed under microscope and measured by morphometric quantification. Results The detection rate of pathological change of grade Ⅲ of heart surface in Xin\|Kang oral liquid groups were lower than in virus control group, the difference between Xin\|Kang medium dose group and virus control group being specially significant ( P <0 01), and the detection rate of severe myocardial damage was much lower. The average area of myocardial damage and area of myocardial damage/area of the whole heart in Xin\|Kang oral liquid groups were markedly lower than those in virus control at the 5th day and the 20th day. Conclusion These results demonstrate that the preventive effect of Xin\|Kang oral liquid on acute Coxsackie B3 viral myocarditis is similar to that of interferon.