非侵袭性与侵袭性基底细胞癌的VEGF表达及微血管密度的研究

The expression of vascular endothelial growth factor and the density of tumoral microvessels in non-invasive and invasive basal cell carcinoma

目的 : 研究非侵袭性基底细胞癌 (non invasivebasalcellcarcinoma ,NIBCC)与侵袭性基底细胞癌 (invasivebasalcellcarcinoma,IBCC)的血管内皮生长因子 (vascularendothelialgrowthfactor,VEGF)的表达和肿瘤微血管密度 (tumoralmicrovasculardensity ,TMVD) ,并探讨两者在BCC生物学行为中的意义。方法 : 应用免疫组化SP法检测 34例BCC石蜡标本组织 (17例非侵袭性 ,17例侵袭性 )中的VEGF、Von Willebrand因子表达。结果 : 在NIBCC与IBCC中 ,VEGF的表达水平中度且无明显统计学差异 (P =0 .189) ;IBCC的TMVD高于NIBCC(P =0 .0 19) ;两者的TMVD、VEGF呈正相关 (rs1 =0 .6 6 7,P =0 .0 0 3;rs2=0 .5 82 ,P =0 .0 14)。结论 : 本实验中VEGF尚不能做为衡量BCC恶性度的指标。TMVD是衡量BCC侵袭性的较好指标之一。

Objective:To investigate the expression of vascular endothelial growth factor (VEGF), the density of tumoral microvessels(TMVD), and their significance in non invasive basal cell carcinoma (NIBCC) and invasive basal cell carcinoma (IBCC).Methods:We used SP immunohistochemical staining for observing VEGF and Von Willebrand factor in NIBCC and IBCCs.Results: There were no statistically significant differences in the moderate level expression of VEGF between NIBCC and IBCC(P=0.189).The level of TMVD in IBCC was higher than that in NIBCC (P=0.019). The expression of VEGF was positively correlated both in NIBCC and IBCCs(r s1 = 0.667 ,P=0.003; r s2 =0.582,P=0.014). Conclusion :Based on this study VEGF might not be taken as an indicator of the malignancy in BCC, while TMVD is a good indicator in assessing the invasiveness of BCC. [