离子束介导大豆DNA小麦后代的蛋白质变异性分析

The Analysis of Protein Variation of Wheat Offsprings Irradiated Exogenous DNA from Soybean

用离子注入法转大豆 DNA至小麦 ,结果表明 ,当代转基因小麦籽粒蛋白质含量有明显变化 ,得到高蛋白 (>19% )和低蛋白含量 (≤ 13.99% )两种极端变异新材料。蛋白质含量大于 19% (对照为 15 .9% )的有 2 2株 ,占处理总株数 (362 6株 )的 0 .7% ;13.99%以下的有 5株 ,占总株数的 0 .1%。DNA导入方式对当代籽粒蛋白质含量无影响 ,无论导入大豆全长 DNA还是片段 DNA ,均引起当代小麦籽粒蛋白质含量发生显著变化 ;

Soybean DNA was transducted into wheat grain with irradiated ways. The results showed that the protein content of transgenic wheat changed obviously, and two new types with high and low protein content extrem variation was obtained. On the basis of it, we analysed the affection of the ways and time of DNA transduction from soybean on protein content of wheat.