小鼠腔前卵泡体外发育研究

Study on development of mouse preantral follicles cultured in vitro.

目的 :通过小鼠腔前卵泡 (preantralfollicles)体外培养 ,探讨白蛋白、血清、FSH对腔前卵泡体外发育的影响。方法 :在培养液中添加白蛋白、血清或血清 +FSH ,观察其对小鼠腔前卵泡体外生长发育的影响。结果 :白蛋白组、血清组、血清+FSH组腔前卵泡体外存活时间分别为 2 .6 4± 1.37d、4.83± 1.6 8d和 6 .72± 3.87d ,3组间有极其显著性差异 (P <0 .0 0 1)。血清 +FSH组中腔前卵泡出现卵泡腔的比率为 14.86 % (2 5 / 16 8) ,排卵率为 17.2 6 % (2 9/ 16 8) ,卵母细胞成熟率为 48.2 8% (14/2 9)。血清组和血清 +FSH组均有卵泡聚集现象。结论 :血清和FSH均能显著延长小鼠腔前卵泡体外存活时间 ,促进生长发育 ,并能引起卵泡的聚集。FSH在卵泡腔的形成中有决定性的作用。

Objective:The effects of BSA,FCS or FSH on mouse preantral follicles development was investigated by culturing in vitro.Methods:The growth and development of mouse preantral follicles cultured in vitro were observed by adding BSA,FCS or FCS plus FSH to the medium.Results:The survival times of follicles cultured in vitro were 2.64±1.37d,4.83±1.68d and 6.72±3.87d respectively.There was a significantly difference between the three groups(P<0.001).During culture,the follicular antrum formed at a rate of 14.86%(25/168),the oocytes ovulated at a rate of 17.26%(29/168) and the oocytes maturated at a rate of 48.28%(14/29)in the medium with FCS plus FSH.The follicular congregation was observed in the medium with FCS or FCSplus FSH.Conclusion:FCS or FSH all significantly prolonged the survival time,promoted the growth and development,and begeted the congregation of follicles cultured in vitro.FSH was a crucial factor in formation of follicular antrum.