TGF-β_1在Dahl高血压大鼠肾小管中的表达

EXPRESSION OF TGF β_1 IN RENAL TUBULAR CELLS OF DAHL HYPERTENSIVE RATS

目的 :探讨 TGF- β1在高血压所致肾损伤过程中的表达规律及意义。方法 :选用 Dahl高血压大鼠 ,5周龄时喂 8%食盐饲料 ,尾套法每周测一次收缩期血压 ,分别取 10周、11周、12周龄大鼠肾脏 ,常规石蜡包埋 ,连续切片 ,SP免疫组织化学法染色检测 TGF- β1的表达 ,免疫组化半定量计数 ,方差分析。结果 :Dahl盐敏感型高血压大鼠皮质肾小管有广泛的 TGF-β1的表达阳性细胞 ,而且随着血压增高TGF- β1的表达增强 ,结论 :TGF-

Objective:Discuss the regulation of TGF β 1 expression and the function in renal injury of Dahl salt sensitive rats.Methods:Dahl salt sensitive and salt resistant rats were fed on 8% NaCl diets at 5 week old.Blood pressure was determined and the kidney were harvested at 10 week、11 week and 12 week old.TGF β 1 assessed by immunohistochemistry.Do analysis of variance after sorting data in the semi ration standard of immunohistochemistry.Results:there are alteration in expression of TGF β 1 in renal cortical tubular cells Dahl salt sensitive rats and increase along with the raising of blood pressure.Conclusion:Extra expression of TGF β 1 may induce the apoptosis of renal tubular and interstitial fibrosis.